Volume 33

Number 4 (2024)

Evaluation of Antibacterial Activities of Aqueous Extract of Black Pepper (Piper nigrum L) Seeds against the Gram Positive Staphylococcus aureus and Gram-Negative Escherichia coli

DOI: https://doi.org/10.XXXXX/mmj.2024.v3304.08

Hossain MA1 , Ahmed SM2 , Zannat KE3 , Afrin A4 , Banu TA5 , Dema TS6 , Nishat EF7 , Ferdous J8 , Nawar A9 , Ferdaus UJ10 , Tanzim SM11

Abstract

Antibiotic resistance (AMR) represents a serious threat to public health and poses challenges in disease prevention and treatment despite various efforts to combat it. Evaluation of the in vitro antibacterial activity of aqueous extracts of black pepper seeds (Piper nigrum L.) against two infectious pathogens: Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The Department of Pharmacology and Therapeutics and the Department of Microbiology of Mymensingh Medical College conducted the study from July 2022 to June 2023. The antibacterial activity of Aqueous black pepper seed extract (ABPE) was evaluated at different doses using disk diffusion and broth dilution methods. The extract was prepared using 10.0% dimethyl sulfoxide (DMSO) and water as solvent. The commonly used antibiotic ciprofloxacin was used in the broth dilution method and the results were compared with those for aqueous extracts. To confirm a more precise range of antimicrobial susceptibility of the extracts, ABPE was used at seven different concentrations (100, 80, 60, 40, 20, 10 and 5 mg/mL). Selected concentrations were then used as needed. ABPE showed an inhibitory effect on the above bacteria at doses of 90 mg/ml and higher. The Minimum inhibitory concentration (MIC) values for Escherichia coli and Staphylococcus aureus were 85 and 90 mg/ml ABPE, respectively. The MIC of ciprofloxacin against Staphylococcus aureus and Escherichia coli was currently 1μg/ml. The MIC of ciprofloxacin was lowest for the organisms tested compared to the MIC of ABPE. This work clearly demonstrates the antibacterial sensitivity of Staphylococcus aureus and Escherichia coli to an aqueous extract of black pepper seeds.

Keywords: Antibacterial effects, Black pepper seeds, Staphylococcus aureus, Escherichia coli, Disc diffusion, Aqueous extract


  1. M Phil (Pharmacology), Final Part Student

    Mymensingh Medical College, Mymensingh

  2. Associate Professor, Department of Pharmacology

    Mymensingh Medical College, Mymensingh

  3. Assistant Professor of Pharmacology

    Diabetic Association Medical College, Faridpur

  4. Lecturer of Pharmacology

    Mymensingh Medical College, Mymensingh

  5. Lecturer, Department of Pharmacology

    Rangpur Medical College, Rangpur

  6. M Phil (Pharmacology), Final Part Student

    Mymensingh Medical College, Mymensingh

  7. Lecturer of Pharmacology

    Netrokona Medical College, Netrokona

  8. Lecturer of Pharmacology

    Community Based Medical College, Mymensingh

  9. M Phil (Pharmacology), Final Part Student

    Mymensingh Medical College, Mymensingh

  10. MPH (Thesis Part) Student, Department of Community Medicine

    Mymensingh Medical College, Mymensingh

  11. Lecturer, Department of Pathology

    Mymensingh Medical College, Mymensingh

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Volume 33, Number 4 (2024)
Page: 1002-1008